Fig. 2. Principal component analysis (PCA) scores plot showing the separation between HL-60 cells (n= 5) total lipidome profile (analysed in positive and negative ion modes) prior and post cryopreservation in cryo-additive agents. HL-60 cells were pre-incubated for 24 hours in cell culture media [5: Blue-sky circles] and cryopreserved in DMSO alone [1: Red circles], DMSO + Nig [2: Blue circles]; in DMSO +Sal [3: Green circles] and cells frozen DMSO + Nig and Sal [4: Yellow circles].